要点：CITE-seq 的膜蛋白marker的意义；批量绘制 FeaturePlot。
- T: CD5, CD7, TNFRSF8(CD30)?, NCAM1(CD56)?
- mono: CD33, ANPEP(CD13), FCGR1A(CD64), FUT4(CD15)?, IL3RA(CD123)
- B: MME(CD10), CD38
- ery: TFRC(CD71),
- HSC: CD34, KIT(CD117),
1,feature plot with no axis
2. Code
FeaturePlot(scObj, features =c("CD3D", "CD8A", "GZMB", "CCR7", "CCL5",
"CD5","CD7",
"HLA-DRA",
"LYZ", "CD33", "ANPEP", "FCGR1A", "IL3RA",
"CD79A","CD79B","VPREB1", "IGJ",
"MME","CD38",
"HBA1", "GATA1", "TFRC",#CD71
"CD34", "SOCS2",
"GAPDH"),
cols = c("lightgrey", 'red'),
ncol = 5 ) & NoLegend() & NoAxes() & theme(
panel.border = element_rect(color = "black", size = 1)
)
